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Image Search Results
Journal: Cell Discovery
Article Title: Spatiotemporal regulation of insulin signaling by liquid–liquid phase separation
doi: 10.1038/s41421-022-00430-1
Figure Lengend Snippet: a In vitro LLPS assay with 20 μM recombinant His-tagged proteins GFP, GSK3β-GFP, PRAS40-GFP, BAD-GFP, and P27-GFP with or without 3% PEG8000. Scale bar, 10 μm. b In vitro LLPS assay with recombinant His-tagged full-length IRS1-GFP with 3% PEG8000 ± NaCl. Scale bar, 10 μm. c Schematic illustration of domains and fragments of mouse IRS1. Eight tyrosine sites regulated by insulin receptor are shown in the diagram. d In vitro LLPS assay with recombinant His-tagged four fragments IRS1-GFP with 3% PEG8000. Scale bar, 10 μm. e Fusion of droplets of His-tagged IRS1 N243-S600 -GFP. Red arrows showed the two droplets that fused during the experimental period. Time Zero started at 90 s after the addition of PEG8000. Scale bar, 10 μm.
Article Snippet: 1,6-Hexanediol was from Sangon Biotech (Cat No. A100159), and
Techniques: In Vitro, Recombinant